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Forensic Analysis of Human DNA
Gordon Research Conference

Advancing Fundamental Technologies from the Crime Lab to the Crime Scene

Dates

June 19-24, 2016

Location

Waterville Valley
Waterville Valley, NH

Organizers

Chairs:
James P. Landers & Joan M. Bienvenue

Vice Chairs:
Robin Cotton & Steven Lee

Meeting Description

Since the development of RFLP as the first crude methodology for forensic DNA fingerprinting by Alec Jeffreys in 1985, the methods for creating court-defensible DNA profiles have evolved tremendously. With that evolution, there has been a corresponding increase in the demands for more rapid analysis with improved specificity and sensitivity. This meeting addresses the challenges facing forensic scientists and technology development scientists focused on sensitive STR profiling from DNA extracted from a broader range of sample types and with increasingly lower copy number. The focus will be on current forensic DNA analysis chemistries and instrumentation used for STR profiling, as well as next gen sequencing platforms that potentially offer enhanced capabilities for generating data for human ID. This will be an attractive discussion environment for stimulating discussions between the scientists and molecular biologists developing the technology, and the analysts using it in the criminal justice system. These discussions will be critical in synchronizing the needs of the analyst with the development capabilities of those in the research and development sector.

The uniqueness of this conference will be the fundamental discussions between user and developer, analyst and scientist, court defenders and data generators, all within a scientific framework defocused from casework scenarios. The diverse perspectives of those in the criminal justice system (lab managers, analysts, prosecutors) and those developing the technology critical to them, will lead to discussions that benefit all, regardless of seniority. These discussions will enlighten us and expand our scope on the utility of the chemistries that currently exist, as well as the areas that need intense focus from the research and development communities, both academic and industrial. The uniqueness of the GRC format, through oral and poster presentations, will assure a discussion experience unlike any available to those in the forensic DNA community with the conferences that exist today.

Contributors

Final Meeting Program

Sunday
2:00 pm - 9:00 pmArrival and Check-in
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmForensic DNA Analysis: Challenges and Triumphs
Discussion Leader: Mark Guilliano (QIAGEN Corporation, USA)
7:40 pm - 8:20 pmJessica McBride (University of Wisconsin-Milwaukee, USA)
"DNA and 'Making a Murderer'"
8:20 pm - 8:35 pmDiscussion
8:35 pm - 9:15 pmNiels Morling (University of Copenhagen, Denmark)
"Forensic Genetics - Quo Vadis?"
9:15 pm - 9:30 pmDiscussion
Monday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmAnalysis of DNA Mixtures
Discussion Leader: Margaret Ewing (Promega Corporation, USA)
9:00 am - 9:35 amCharles Brenner (University of California, Berkeley, USA)
"Re-Thinking Cliches: Traditional Errors in Mixture and Haplotype Evidence Calculations"
9:35 am - 9:55 amDiscussion
9:55 am - 10:20 amGroup Photo / Coffee Break
10:20 am - 10:55 amCatherine Grgicak (Boston University School of Medicine, USA)
"Forensic DNA Research, Validation and Pedagogy Using Empirical and Simulated Data: Understanding the Behavior of Mixtures"
10:55 am - 11:15 amDiscussion
11:15 am - 11:50 amMichael Coble (National Institute of Standards and Technology, USA)
"The Present (and Future) State of DNA Mixture Interpretation"
11:50 am - 12:10 pmDiscussion
12:10 pm - 12:30 pmPoster Previews
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmHuman Factors and Contextual Bias
Discussion Leader: Melissa Taylor (National Institute of Standards and Technology, USA)
7:30 pm - 8:10 pmCecelia Crouse (Palm Beach County Sheriff's Office, USA)
"The Loss of Critical Thinking in the 21st Century Forensic DNA Laboratory: Confronting the 'Next Gen' Challenges"
8:10 pm - 8:30 pmDiscussion
8:30 pm - 9:10 pmScott Shappell (Embry-Riddle Aeronautical University, USA)
"Shiftwork- and Fatigue-Induced Bias in Interpreting Forensic Data"
9:10 pm - 9:30 pmDiscussion
Tuesday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmNucleic Acid Interrogation for Body Fluid Identification
Discussion Leader: Rick Tontarski (Defense Forensic Science Center, USA)
9:00 am - 9:35 amSarah Seashols (Virginia Commonwealth University, USA)
"Exploring the Utility of miRNAs as Biomarkers for Body Fluids"
9:35 am - 9:55 amDiscussion
9:55 am - 10:20 amCoffee Break
10:20 am - 10:55 amTitia Sijen (Netherlands Forensic Institute, The Netherlands)
"mRNA-Based Cell and Organ Typing in a Forensic Context"
10:55 am - 11:15 amDiscussion
11:15 am - 11:50 amSally Harbison (Institute of Environmental Science and Research Limited, New Zealand)
"Considerations for the Implementation of mRNA in Forensic Casework"
11:50 am - 12:10 pmDiscussion
12:10 pm - 12:30 pmPoster Previews
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmDNA Typing for Familial Linkage
Discussion Leader: Lucy Davis (LDH Consultants, USA)
7:30 pm - 8:10 pmKenneth Kidd (Yale University, USA)
"Microhaplotypes in Forensics: Transiting from Theory to Application"
8:10 pm - 8:30 pmDiscussion
8:30 pm - 9:10 pmArnoud Kal (Netherlands Forensic Institute, The Netherlands)
"Application of Kinship Analysis in Familial Searching and Disaster Victim Identification"
9:10 pm - 9:30 pmDiscussion
Wednesday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmNext Gen Tech for DNA Analysis
Discussion Leader: Bruce McCord (Florida International University, USA)
9:00 am - 9:35 amPeter Vallone (National Institute of Standards and Technology, USA)
"Uncovering Sequence Variation Within STRs by NGS"
9:35 am - 9:55 amDiscussion
9:55 am - 10:20 amCoffee Break
10:20 am - 10:55 amMarie Allen (Uppsala University, Sweden)
"Next Generation Sequencing of Degraded Forensic and Historical Samples"
10:55 am - 11:15 amDiscussion
11:15 am - 11:50 amAakrosh Ratan (University of Virginia, USA)
"Uncovering Human Structural Variation Using Next Generation Sequencing"
11:50 am - 12:10 pmDiscussion
12:10 pm - 12:30 pmPoster Previews
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
7:30 pm - 9:30 pmBioinformatics and Probabilistic Genotyping
Discussion Leader: Kimberly Andreaggi (ARP Sciences / Armed Forces DNA Identification Laboratory (AFDIL), USA)
7:30 pm - 8:10 pmCraig O'Connor (Office of Chief Medical Examiner of the City of New York, USA)
"Integrating Probabilistic Genotyping into a Laboratory"
8:10 pm - 8:30 pmDiscussion
8:30 pm - 9:10 pmCharlotte Word (Word Consulting, USA)
"Probabilistic Genotyping: Issues and Research Needs"
9:10 pm - 9:30 pmDiscussion
Thursday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmTrace DNA Analysis
Discussion Leader: Minh Nyugen (National Institute of Justice, U.S. Department of Justice, USA)
9:00 am - 9:35 amR.A.H. van Oorschot (Forensic Services Department, Victoria Police, Australia)
"DNA Transfer: Where From and Where To"
9:35 am - 9:55 amDiscussion
9:55 am - 10:20 amCoffee Break
10:20 am - 10:55 amTodd Bille (Bureau of Alcohol, Tobacco, Firearms and Explosives (ATF), USA)
"Important Aspects of Cell-Free Nucleic Acids"
10:55 am - 11:15 amDiscussion
11:15 am - 11:50 amMechthild Prinz (John Jay College of Criminal Justice, USA)
"Trace DNA Transfer and Probative Value"
11:50 am - 12:10 pmDiscussion
12:10 pm - 12:30 pmPoster Previews
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 5:30 pmPoster Session
5:30 pm - 7:30 pmSingle Nucleotide Polymorphisms for Phenotyping
Discussion Leader: Katherine Gettings (National Institute of Standards and Technology, USA)
5:30 pm - 6:10 pmDaniele Podini (George Washington University, USA)
"Microhaplotype for Mixture Deconvolution and Ancestry Inference"
6:10 pm - 6:30 pmDiscussion
6:30 pm - 7:10 pmSusan Walsh (Indiana University - Purdue University Indianapolis, USA)
"Predictive Biometrics and the Human Face"
7:10 pm - 7:30 pmDiscussion
8:00 pmDinner
Friday
7:30 am - 8:30 amBreakfast
9:00 amDeparture
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