1999 Bioenergetics Conference GRC

(S, pm)	Mechanisms in generation and consumption of Mitchell protons
Discussion Leader: Ron Kaplan
7:45 - 8:00	Ron Kaplan "Overview of molecular mechanisms in proton translocation"
8:00 - 8:30	Janos Lanyi, University of California - Irvine, USA "Structure of bacteriorhodopsin by x-ray diffraction and its implications for the mechanism of proton translocation"
8:30 - 9:00	Keith Garlid, Oregon Institute of Science & Technology, USA "The mechanism of uncoupling by uncoupling proteins"
9:00 - 9:30	J. Baz Jackson, University of Birmingham, UK "Structure-function relationships of H⁺-translocating transhydrogenase".
9:30 - 10:00	Short report on transhydrogenase by J. Rydström (5 min) followed by general discussion.
(M, am)	Structure and Mechanism of F₁-ATPases
Discussion Leader: Masamitsu Futai
8:45 - 9:00	Masamitsu Futai, Osaka University, Japan "Overview of structure-function relationships in F₁-ATPases".
9:00 - 9:30	John Walker, MRC Laboratory of Molecular Biology, Cambridge, UK "Structural states in the catalytic cycle of F₁-ATPase"
9:30 - 10:00	Masasuke Yoshida, Institute of Technology, Japan "Mechanocoupling in ATP synthase".
10:00 - 10:25	Short reportson mapping the route of γ during rotation by C. Montemagno (5 min) followed by general discussion.
10:45 -.11:15	Alan Senior, University of Rochester, USA "Will the real catalytic mechanism of F₁F₀ please come forward?"
11:15 - 11:45	Richard Cross, SUNY Health Center at Syracuse, USA "Subunit rotation in F₀F₁-ATP synthase".
11:45 - 12:15	Peter Pedersen, Johns Hopkins School of Medicine, USA "Insights into the chemical mechanism of ATP synthase".
12:15 - 12:30	General discussion
(M, pm)	Mechanism and regulation of V-ATPases
Discussion Leader: Barry Bowman
7:30 - 7:45	Barry Bowman, University of California - Santa Cruz, USA "Overview of the V-ATPases".
7:45 - 8:15	Michael Forgac, Tufts University School of Medicine, USA "Structure and properties of the bovine coated vesicle and yeast vacuolar ATPases".
8:15 - 8:45	Patricia Kane, SUNY Health Center at Syracuse, USA "Regulation of the yeast vacuolar H⁺-ATPase".
8:45 - 9:15	Helmut Wieczorek, University of Osnabrück, Germany "The insect V-ATPase: Structural and regulatory aspects".
9:15 - 9:30	Gerhard Grüber "Molecular architecture of Manuca sexta midgut V₁-ATPase".
9:30 - 10:15	General Discussion
(Tu, am)	Structure and Function of the F₀ component of ATP Synthase
Discussion Leader: Robert Fillingame
8:45 - 9:00	Robert Fillingame, University of Wisconsin, USA "Overview of the structure and function of F₀"
9:00 - 9:30	Marc Girvin, Albert Einstein College of Medicine, USA "Structure and conformational changes of subunit c of the F₁F₀ ATP synthase"
9:30 - 10:00	Georg Groth, University of Düsseldorf, Germany "Molecular models of the membrane-integrated c subunit oligomer in F-ATPases"
10:00 - 10:30	Short report on evidence supporting 5 transmembrane α-helices subunit a by S. Vik (5 min) followed by general discussion
10:45 - 11:15	Peter Dimroth, ETH Zurich, Switzerland "Voltage-regulated torque drives the motor of the ATP synthase"
11:15 - 11:45	Wolfgang Junge, University of Osnabrück, Germany "Stepped rotation and elastic energy storage in ATP synthase"
11:45 - 12:30	Short report on the subunits of yeast F₀ by J. Velours (5 min) followed by general discussion
(Tu, pm)	Structure-Function Relationships in Cytochrome c Oxidase
Discussion Leader: Shelagh Ferguson-Miller
7:30 - 7:45	Shelagh Ferguson-Miller, Michigan State University, USA "An overview of cytochrome c oxidase"
7:45 - 8:15	Hartmut Michel, Max Planck Institute for Biophysics, Frankfurt, Germany "Cytochrome c oxidase: coupling of the catalytic cycle to proton pumping by electrostatic repulsion"
8:15 - 8:45	Gerald Babcock, Michigan State University, USA "O₂ reduction by cytochrome c oxidase"
8:45 - 9:15	Peter Brzezinski, Chalmer University, Sweden "Electron-proton interactions in terminal oxidases"
9:15 - 9:45	Bernhard Kadenbach, Philipps University, Germany "Control of respiration and energy transduction in eukaryotic cytochrome c oxidase"
9:45 - 10:00	General discussion.
(W, am)	Structure-Function Relationships in the bc₁ Complex
Discussion Leader: Thomas Link
8:45 - 9:00	Thomas Link, Uniklinikum Frankfurt, Germany "Overview of the structure and function of the bc₁ complex"
9:00 - 9:30	So Iwata, Uppsala University, Sweden "Structural basis of the protonmotive Q-cycle: the complete structure of the cytochrome bc₁ complex"
9:30 - 9:45	Comments from E. Berry and C-A. Yu on the bc₁ complex
9:45 - 10:15	Fevzi Daldal, University of Pennsylvania, USA "Structure, function and biogenesis of the cytochrome bc₁ complex from Rhodobacter capsulatus"
10:15 - 10:35	Short report on the bc₁ complex from yeast by C. Hunte (8 min) followed by General discussion
(W, am)	Structure and Function of Complex I
Discussion Leader: Immo Scheffler
10:50 - 11:05	Immo Scheffler, University of California - San Diego, USA "An overview of problems associated with complex I"
11:05 - 11:35	Thorsten Friedrich, University of Düsseldorf, Germany "A novel redox group in complex I"
11:35 - 12:05	Takao Yagi, Scripps Research Institute, USA "Progress on understanding complex I from bacteria to mammals"
12:05 - 12:30	A short report on pierecidin resistant mutants by A. Dupuis (5 min) followed by general discussion.
(W, pm)	Molecular Mechanisms of Work Performing ATPases
Discussion Leader: Peter Pedersen
7:30 - 7:45	Peter Pedersen, Johns Hopkins School of Medicine, USA "An overview of workhorse ATPases"
7:45 - 8:15	C. Stauffacher "Structure of the ABC of the Rbs. ABC transporter"
8:15 - 8:45	Smita Patel, Ohio State University "Structure and mechanism of T₇ DNA helicase"
8:45 - 9:15	Barry Rosen, Wayne State University "The bacterial ArsA ATPase"
9:15 - 10:00	general discussion.
(Th, am)	Concurrent Panel Discussions
Panel Discussion A: Structure and Function of the Stalks of ATP Synthase
Discussion Leader: Roderick Capaldi
8:45 - 9:00	Roderick Capaldi, University of Oregon, USA An overview of the rotor and stator stalks of E. coli ATP synthase.
9:00 - 10:15	Panelists: Robert Aggelar, University of Oregon, USA Brian Cain, University of Florida, USA Stanley Dunn, University of Western Ontario, Canada Robert Nakamoto, University of Virginia, USA H. Lill (15 min each with 5 min devoted to discussion).
10:15-10:50	general discussion.
Panel Discussion B: On the Rate Determining Step of the bc₁ Complex and the Mechanism of H⁺ Translocation in Cytochrome c Oxidase
Discussion Leader: Robert Gennis
8:45 - 9:00	Robert Gennis, University of Illinois An overview of the outstanding problems in the electron transport complexes
9:00 - 9:55	Invited discussants on the bc₁ complex: Ulrich Brandt, Uniklinikum Frankfurt, Germany Anthony Crofts, University of Illinois, USA Bernie Trumpower, Dartmouth College, USA (15 min. each with 5 min. devoted to discussion).
9:55 - 10:50	Invited discussants on cytochrome c oxidase: M. Verkhovsky Peter Rich, University College, London, UK Shinya Yoshikawa, Himeiji Institute of Technology, Japan (15 min each with 5 min devoted to discussion).
(Th, am)	Concurrent Workshops
11:10 - 12:30	Workshop A: F₀F₁ ATP synthase and ATPases
Discussion Leaders: Steven Vik, Southern Methodist University, USA Emma Jean Bowman, University of California - Santa Cruz, USA
11:10 - 12:30	Workshop B: Electron transport and ion transport systems.
Discussion Leaders: P. Leslie Dutton, University of Pennsylvania Alexander Konstantinov, Moscow State University, Russia
(Th, pm)	Late Breaking Crystal Structures
Discussion Leader: B. Ackrell
7:45 - 7:55	B. Ackrell, Overview of complex II
7:55 - 8:20	T. Iverson, Structure of intact E. coli fumarate reductase complex
8:20 - 8:45	C.R. Lancaster, Recent progress on the structure determination of fumarate dehydrogenase from Wolinella succinogenes
8:45 - 9:00	general discussion.
(Th, pm)	The F₀ and F₁ Molecular Motors in Motion
Discussion Leader: W. Cramer
9:00 - 9:40	G. Oster, Mechanochemistry of F₁ and F₀

(S, pm)

Mechanisms in generation and consumption of Mitchell protons

Discussion Leader: Ron Kaplan

7:45 - 8:00

Ron Kaplan
"Overview of molecular mechanisms in proton translocation"

8:00 - 8:30

Janos Lanyi, University of California - Irvine, USA
"Structure of bacteriorhodopsin by x-ray diffraction and its implications for the mechanism of proton translocation"

8:30 - 9:00

Keith Garlid, Oregon Institute of Science & Technology, USA
"The mechanism of uncoupling by uncoupling proteins"

9:00 - 9:30

J. Baz Jackson, University of Birmingham, UK
"Structure-function relationships of H⁺-translocating transhydrogenase".

9:30 - 10:00

Short report on transhydrogenase by J. Rydström (5 min) followed by general discussion.

(M, am)

Structure and Mechanism of F₁-ATPases

Discussion Leader: Masamitsu Futai

8:45 - 9:00

Masamitsu Futai, Osaka University, Japan
"Overview of structure-function relationships in F₁-ATPases".

9:00 - 9:30

John Walker, MRC Laboratory of Molecular Biology, Cambridge, UK
"Structural states in the catalytic cycle of F₁-ATPase"

9:30 - 10:00

Masasuke Yoshida, Institute of Technology, Japan
"Mechanocoupling in ATP synthase".

10:00 - 10:25

Short reportson mapping the route of γ during rotation by C. Montemagno (5 min) followed by general discussion.

10:45 -.11:15

Alan Senior, University of Rochester, USA
"Will the real catalytic mechanism of F₁F₀ please come forward?"

11:15 - 11:45

Richard Cross, SUNY Health Center at Syracuse, USA
"Subunit rotation in F₀F₁-ATP synthase".

11:45 - 12:15

Peter Pedersen, Johns Hopkins School of Medicine, USA
"Insights into the chemical mechanism of ATP synthase".

12:15 - 12:30

General discussion

(M, pm)

Mechanism and regulation of V-ATPases

Discussion Leader: Barry Bowman

7:30 - 7:45

Barry Bowman, University of California - Santa Cruz, USA
"Overview of the V-ATPases".

7:45 - 8:15

Michael Forgac, Tufts University School of Medicine, USA
"Structure and properties of the bovine coated vesicle and yeast vacuolar ATPases".

8:15 - 8:45

Patricia Kane, SUNY Health Center at Syracuse, USA
"Regulation of the yeast vacuolar H⁺-ATPase".

8:45 - 9:15

Helmut Wieczorek, University of Osnabrück, Germany
"The insect V-ATPase: Structural and regulatory aspects".

9:15 - 9:30

Gerhard Grüber
"Molecular architecture of Manuca sexta midgut V₁-ATPase".

9:30 - 10:15

General Discussion

(Tu, am)

Structure and Function of the F₀ component of ATP Synthase

Discussion Leader: Robert Fillingame

8:45 - 9:00

Robert Fillingame, University of Wisconsin, USA
"Overview of the structure and function of F₀"

9:00 - 9:30

Marc Girvin, Albert Einstein College of Medicine, USA
"Structure and conformational changes of subunit c of the F₁F₀ ATP synthase"

9:30 - 10:00

Georg Groth, University of Düsseldorf, Germany
"Molecular models of the membrane-integrated c subunit oligomer in F-ATPases"

10:00 - 10:30

Short report on evidence supporting 5 transmembrane α-helices subunit a by S. Vik (5 min) followed by general discussion

10:45 - 11:15

Peter Dimroth, ETH Zurich, Switzerland
"Voltage-regulated torque drives the motor of the ATP synthase"

11:15 - 11:45

Wolfgang Junge, University of Osnabrück, Germany
"Stepped rotation and elastic energy storage in ATP synthase"

11:45 - 12:30

Short report on the subunits of yeast F₀ by J. Velours (5 min) followed by general discussion

(Tu, pm)

Structure-Function Relationships in Cytochrome c Oxidase

Discussion Leader: Shelagh Ferguson-Miller

7:30 - 7:45

Shelagh Ferguson-Miller, Michigan State University, USA
"An overview of cytochrome c oxidase"

7:45 - 8:15

Hartmut Michel, Max Planck Institute for Biophysics, Frankfurt, Germany
"Cytochrome c oxidase: coupling of the catalytic cycle to proton pumping by electrostatic repulsion"

8:15 - 8:45

Gerald Babcock, Michigan State University, USA
"O₂ reduction by cytochrome c oxidase"

8:45 - 9:15

Peter Brzezinski, Chalmer University, Sweden
"Electron-proton interactions in terminal oxidases"

9:15 - 9:45

Bernhard Kadenbach, Philipps University, Germany
"Control of respiration and energy transduction in eukaryotic cytochrome c oxidase"

9:45 - 10:00

General discussion.

(W, am)

Structure-Function Relationships in the bc₁ Complex

Discussion Leader: Thomas Link

8:45 - 9:00

Thomas Link, Uniklinikum Frankfurt, Germany
"Overview of the structure and function of the bc₁ complex"

9:00 - 9:30

So Iwata, Uppsala University, Sweden
"Structural basis of the protonmotive Q-cycle: the complete structure of the cytochrome bc₁ complex"

9:30 - 9:45

Comments from E. Berry and C-A. Yu on the bc₁ complex

9:45 - 10:15

Fevzi Daldal, University of Pennsylvania, USA
"Structure, function and biogenesis of the cytochrome bc₁ complex from Rhodobacter capsulatus"

10:15 - 10:35

Short report on the bc₁ complex from yeast by C. Hunte (8 min) followed by General discussion

(W, am)

Structure and Function of Complex I

Discussion Leader: Immo Scheffler

10:50 - 11:05

Immo Scheffler, University of California - San Diego, USA
"An overview of problems associated with complex I"

11:05 - 11:35

Thorsten Friedrich, University of Düsseldorf, Germany
"A novel redox group in complex I"

11:35 - 12:05

Takao Yagi, Scripps Research Institute, USA
"Progress on understanding complex I from bacteria to mammals"

12:05 - 12:30

A short report on pierecidin resistant mutants by A. Dupuis (5 min) followed by general discussion.

(W, pm)

Molecular Mechanisms of Work Performing ATPases

Discussion Leader: Peter Pedersen

7:30 - 7:45

Peter Pedersen, Johns Hopkins School of Medicine, USA
"An overview of workhorse ATPases"

7:45 - 8:15

C. Stauffacher
"Structure of the ABC of the Rbs. ABC transporter"

8:15 - 8:45

Smita Patel, Ohio State University
"Structure and mechanism of T₇ DNA helicase"

8:45 - 9:15

Barry Rosen, Wayne State University
"The bacterial ArsA ATPase"

9:15 - 10:00

general discussion.

(Th, am)

Concurrent Panel Discussions

Panel Discussion A: Structure and Function of the Stalks of ATP Synthase

Discussion Leader: Roderick Capaldi

8:45 - 9:00

Roderick Capaldi, University of Oregon, USA
An overview of the rotor and stator stalks of E. coli ATP synthase.

9:00 - 10:15

Panelists:

Robert Aggelar, University of Oregon, USA
Brian Cain, University of Florida, USA
Stanley Dunn, University of Western Ontario, Canada
Robert Nakamoto, University of Virginia, USA
H. Lill

(15 min each with 5 min devoted to discussion).

10:15-10:50

general discussion.

Panel Discussion B: On the Rate Determining Step of the bc₁ Complex and the Mechanism of H⁺ Translocation in Cytochrome c Oxidase

Discussion Leader: Robert Gennis

8:45 - 9:00

Robert Gennis, University of Illinois
An overview of the outstanding problems in the electron transport complexes

9:00 - 9:55

Invited discussants on the bc₁ complex:

Ulrich Brandt, Uniklinikum Frankfurt, Germany
Anthony Crofts, University of Illinois, USA
Bernie Trumpower, Dartmouth College, USA

(15 min. each with 5 min. devoted to discussion).

9:55 - 10:50

Invited discussants on cytochrome c oxidase:

M. Verkhovsky
Peter Rich, University College, London, UK
Shinya Yoshikawa, Himeiji Institute of Technology, Japan

(15 min each with 5 min devoted to discussion).

(Th, am)

Concurrent Workshops

11:10 - 12:30

Workshop A: F₀F₁ ATP synthase and ATPases

Discussion Leaders:
Steven Vik, Southern Methodist University, USA
Emma Jean Bowman, University of California - Santa Cruz, USA

11:10 - 12:30

Workshop B: Electron transport and ion transport systems.

Discussion Leaders:
P. Leslie Dutton, University of Pennsylvania
Alexander Konstantinov, Moscow State University, Russia

(Th, pm)

Late Breaking Crystal Structures

Discussion Leader: B. Ackrell

7:45 - 7:55

B. Ackrell,
Overview of complex II

7:55 - 8:20

T. Iverson,
Structure of intact E. coli fumarate reductase complex

8:20 - 8:45

C.R. Lancaster,
Recent progress on the structure determination of fumarate dehydrogenase from Wolinella succinogenes

8:45 - 9:00

general discussion.

(Th, pm)

The F₀ and F₁ Molecular Motors in Motion

Discussion Leader: W. Cramer

9:00 - 9:40

G. Oster,
Mechanochemistry of F₁ and F₀

Bioenergetics

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Archaea: Ecology, Metabolism and Molecular Biology

Bacterial Cell Surfaces

Mechanisms of Membrane Transport

Metallocofactors

Mitochondria and Chloroplasts

Mitochondria in Health and Disease

Molecular Membrane Biology

Photosynthesis

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