Saturday
2:00 pm - 5:00 pm
Arrival and Check-in
3:30 pm - 3:45 pm
Introductory Comments by GRC Site Staff / Welcome from the GRS Chair
3:45 pm - 4:30 pm
Keynote Session: Single Molecule Approaches to Intracellular Kinetics
Discussion Leader: Xiaoyu Shi (University of California, San Francisco, USA)
3:45 pm - 4:15 pm
Johan Elf (Uppsala University, Sweden)
"Single Molecule Approaches to Intracellular Kinetics"
4:15 pm - 4:30 pm
Discussion
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
New and Notable Single Molecule Methods
Discussion Leader: Anna-Karin Gustavsson (Stanford University, USA)
7:30 pm - 7:50 pm
Mingjie Dai (Harvard Medical School, USA)
"Single-Molecule Protein Identification Through Peptide Chain Linear Barcoding and Optical Readout"
7:50 pm - 8:00 pm
Discussion
8:00 pm - 8:20 pm
Michael Lawson (California Institute of Technology, USA)
"Characterizing Complex Phenotypes of a Library of Cells at Single Molecule Sensitivity"
8:20 pm - 8:30 pm
Discussion
8:30 pm - 8:50 pm
Florian Schueder (Ludwig Maximilian University of Munich / Max Planck Institute of Biochemistry, Germany)
"Multiplexed 3D Super-Resolution Imaging of Whole Cells Using Spinning Disk Confocal Microscopy and DNA-PAINT"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:20 pm
Tiancheng Zuo (University of Michigan, USA)
"Single-Molecule Polarization-Resolved Microscopy of Nanoparticle-Coupled Dye Molecules"
9:20 pm - 9:30 pm
Discussion
Sunday
7:30 am - 8:30 am
Breakfast
9:00 am - 11:00 am
Applications of Single Molecule Methods and Superresolution Microscopy
Discussion Leader: Ye Tao (Rowland Institute, Harvard University, USA)
9:00 am - 9:20 am
Salome Veshaguri (University of Copenhagen, Denmark)
"Single Molecule Measurements of Substrate Transport by a Primary Active Proton Pumping ATPase"
9:20 am - 9:30 am
Discussion
9:30 am - 9:50 am
Kathy Chaurasiya (Leiden University, The Netherlands)
"Visualizing the Mechanism of H-NS-Mediated Gene Regulation"
9:50 am - 10:00 am
Discussion
10:00 am - 10:20 am
Shih-Chin Wang (Johns Hopkins School of Medicine, USA)
"A Single-Molecule Blood Test for Mutant Tumor Proteins"
10:20 am - 10:30 am
Discussion
10:30 am - 10:50 am
Michael Morse (Northeastern University, USA)
"Binding and Conformational Change of APOBEC3 Family Proteins Probed Using Force Spectroscopy"
10:50 am - 11:00 am
Discussion
11:00 am - 12:30 pm
Poster Session
Coffee will be served in the poster area from 11:00 am - 11:30 am
12:30 pm - 1:30 pm
Lunch
1:30 pm - 2:30 pm
Mentorship Component: Avoid Pitfalls in Early Career Research
Discussion Leader: Jorine Eeftens (Princeton University, USA)
1:30 pm - 2:30 pm
Panel Discussion
Avoid Pitfalls in Early Career Research
Johan Elf (Uppsala University, Sweden)
Ye Tao (Rowland Institute, Harvard University, USA)
Valda Vinson (Science, AAAS, USA)
2:30 pm - 3:00 pm
Evaluation Period
Fill in GRS Evaluation Forms
3:00 pm
Seminar Concludes