Friday
12:00 pm - 4:00 pm
Arrival and Check-in
2:30 pm - 4:00 pm
Poster Session
4:00 pm - 4:15 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
4:15 pm - 6:00 pm
Expanding the Chemical Space of Metalloenzymes
4:15 pm - 4:20 pm
Introduction by Discussion Leader
4:20 pm - 4:35 pm
"Chemical Modification of Complex Fe–S Clusters for Novel Spectroscopic Studies"
4:35 pm - 4:40 pm
Discussion
4:40 pm - 4:55 pm
"Zinc Fingers are a General Target of H2 S for Persulfidation"
4:55 pm - 5:00 pm
Discussion
5:00 pm - 5:15 pm
"Using the Natural Protein, Lanmodulin (LanM), to Overcome the Subtle Chemical Differences Among the Rare Earth Elements"
5:15 pm - 5:20 pm
Discussion
5:20 pm - 5:35 pm
"Aziridine Formation Enabled by Non-Heme Iron Enzymes: Identification and Mechanistic Study"
5:35 pm - 5:40 pm
Discussion
5:40 pm - 5:55 pm
"Mechanistic Interrogations of the Azidation Reaction in Fe(II)/2-Oxoglutarate-Dependent Halogenases"
5:55 pm - 6:00 pm
Discussion
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Homeostasis and Regulation of Metals in Biology
7:30 pm - 7:35 pm
Introduction by Discussion Leader
7:35 pm - 7:50 pm
"Identification of a Biosynthetic Pathway for a Novel Lanthanide-Chelator in Methylobacterium extorquens AM1"
7:50 pm - 7:55 pm
Discussion
7:55 pm - 8:10 pm
"Elucidating the Mechanism of Oxygen Sensing in the Globin Coupled Sensor Proteins"
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:30 pm
"A Tale of Two RNA-Binding Zinc Finger Proteins: Linking Metalation to Reactivity and Function"
8:30 pm - 8:35 pm
Discussion
8:35 pm - 8:45 pm
"Uncovering the Molecular Mechanisms Linking Iron Misregulation and Diabetes"
8:45 pm - 8:50 pm
Discussion
8:50 pm - 9:05 pm
"In vitro Maturation of Nickel Superoxide Dismutase Reveals a Role for Cytoplasmic Histidine in Processing and Metalation"
9:05 pm - 9:10 pm
Discussion
9:10 pm - 9:25 pm
"Characterization of a Novel Methanogen Radical SAM/SPASM Domain Enzyme"
9:25 pm - 9:30 pm
Discussion
Saturday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
Electronic Insights from Spectroscopy, Computation, and Structure
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:30 am
"Non-Innocent Sulfur in Organometallic Chemistry of Fe–S Clusters"
9:30 am - 9:35 am
Discussion
9:35 am - 9:50 am
"Spectroscopic Quantification of O2 Isotopologues by EPR: a Method of Investigating Water Oxidation Catalyst Mechanisms"
9:50 am - 9:55 am
Discussion
9:55 am - 10:15 am
"Resolving Methane Oxidation: and XES Study of Methane Monooxygenase and Fe Model Compounds"
10:15 am - 10:20 am
Discussion
10:20 am - 10:50 am
Coffee Break
10:50 am - 11:05 am
"Pyruvate Formate-Lyase Activating Enzyme: A Catalytically Competent 5'-Deoxyadenosyl Radical Intermediate Abstracting a H-atom from a Natural Substrate Mimic"
11:05 am - 11:10 am
Discussion
11:10 am - 11:25 am
"Towards Time Resolved Structural Studies of Tryptophan 2,3-dioxygenase"
11:25 am - 11:30 am
Discussion
11:30 am - 11:45 am
"Probing O2 Activation and In Vivo Metalation of a Mn/Fe Oxidase"
11:45 am - 11:50 am
Discussion
11:50 am - 12:05 pm
"Examination of the Conformation – Electronic Structure – Product Relationship in Non-Canonical Heme Oxygenase Catalyzed Reactions"
12:05 pm - 12:10 pm
Discussion
12:10 pm - 12:25 pm
"Using Advanced EPR Spectroscopy to Map out the Histidine-Brace in Lytic Polysaccharide Monooxygenases"
12:25 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:15 pm - 6:00 pm
Synthetic Inorganic Chemistry as a Window into Biology
4:15 pm - 4:20 pm
Introduction by Discussion Leader
4:20 pm - 4:35 pm
"Synthetic Fe–S Clusters Supported by Sterically Encumbering Ligands"
4:35 pm - 4:40 pm
Discussion
4:40 pm - 4:55 pm
"Significance of Intramolecular Hydrogen Bonding on the Properties and Reactivity of the Mn(III)-Hydroxo Complexes"
4:55 pm - 5:00 pm
Discussion
5:00 pm - 5:15 pm
"Nitrite Reduction Catalyzed by a Nonheme Fe(II)-Nitro Complex"
5:15 pm - 5:20 pm
Discussion
5:20 pm - 5:35 pm
"Temperature Dependent C-S versus C-OH Bond Formation by Nonheme FeIII(OH)(SAr) Complexes: Relevance to Isopenicilin N Synthase (IPNS)"
5:35 pm - 5:40 pm
Discussion
5:40 pm - 5:55 pm
"Reactive High-spin Iron(IV)-oxo Sites through Dioxygen Activation in a Metal–organic Framework"
5:55 pm - 6:00 pm
Discussion
6:00 pm - 7:00 pm
Dinner
7:00 pm - 7:30 pm
Evaluation Period
Complete the GRS Evaluation Forms; Election of Future Chair(s)
7:30 pm - 9:30 pm
Repurposing Nature: From Design to Evolution
7:30 pm - 7:35 pm
Introduction by Discussion Leader
7:35 pm - 7:50 pm
"Switchable Artificial Metalloproteins to Probe Conformational Control in Enzyme Function"
7:50 pm - 7:55 pm
Discussion
7:55 pm - 8:10 pm
"Peroxygenation and Oxidative Functionalization by Artificial Cu Proteins (ArCuPs)"
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:25 pm
"Design Principle for Site-Selective Hydroxylation in a Pair of Rieske Oxygenases"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:45 pm
"Computational Design of Metal Centers Along De Novo Protein Interfaces"
8:45 pm - 8:50 pm
Discussion
8:50 pm - 9:05 pm
"Hydrophobic Environments Govern Mn-dependent versus Ni-dependent Activity of Quercetin 2,4-dioxygenase"
9:05 pm - 9:10 pm
Discussion
9:10 pm - 9:25 pm
"Structural and Mechanistic Determinants of ddhNTP Catalysis by the Ancient Antiviral Enzyme, Viperin"
9:25 pm - 9:30 pm
Discussion
Sunday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Mechanisms in Metalloenzymology
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:25 am
"Paradigm Shift on the Evolution of Multiheme Cytochromes: Grafting and Pruning of Protein Modules Across Time"
9:25 am - 9:30 am
Discussion
9:30 am - 9:50 am
"Metalloenzyme-Catalyzed Transformations during the Biosynthesis of 3-Thia-a-Amino Acids"
9:50 am - 9:55 am
Discussion
9:55 am - 10:15 am
"Influences of the Outer Coordination Sphere on Cytochrome P460 Structure and Function"
10:15 am - 10:20 am
Discussion
10:20 am - 10:50 am
Coffee Break
10:50 am - 11:05 am
"Non-Canonical Iron-Sulfur Clusters in an Electron Bifurcating Hydrogenase"
11:05 am - 11:10 am
Discussion
11:10 am - 11:25 am
"Primary C-H Bond Hydroxylation at a Heterobimetallic Fe/Mn Cofactor"
11:25 am - 11:30 am
Discussion
11:30 am - 11:45 am
"Radical-Polar Crossover Mechanism of Ethylene Forming Enzyme (EFE) and Rational Perturbation of its Two Branch Points"
11:45 am - 11:50 am
Discussion
11:50 am - 12:05 pm
"How Substrate and Anion Binding Control Reactivity in Non-heme Iron Halogenases"
12:05 pm - 12:10 pm
Discussion
12:10 pm - 12:25 pm
"Polysaccharide Monooxygenase MtPMO9E Requires a Distant Tyrosine Resides to Activate Molecular Oxygen"
12:25 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:15 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Keynote Session
Joint Session with the Metals in Biology GRC
9:30 pm - 11:00 pm
Poster Session
Joint Poster Session with the Metals in Biology GRC
Monday
7:30 am - 8:30 am
Breakfast
9:00 am
Seminar Concludes