Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Site Staff / Welcome from the GRC Chair
7:40 pm - 9:30 pm
Keynote Session: The Topoisomerase World from Phylogeny to Molecular Structures
Discussion Leader: Mary-Ann Bjornsti (University of Alabama at Birmingham, USA)
7:40 pm - 7:50 pm
Introduction by Discussion Leader
7:50 pm - 8:25 pm
Patrick Forterre (Institut Pasteur, France)
"Evolution and Diversity of Topoisomerases"
8:25 pm - 8:40 pm
Discussion
8:40 pm - 9:15 pm
Nancy Kleckner (Harvard University, USA)
"Type II Topoisomerases as Mediators of Mechanical Stress"
9:15 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
Antibacterial Drugs Targeting Topoisomerases and Antibiotic Resistance
Discussion Leader: Mark Fisher (St. George's, University of London, United Kingdom)
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:40 am
Anthony Maxwell (John Innes Centre, United Kingdom)
"Microcin B17, a Microbial Toxin that Targets DNA Gyrase: Activity, Biosynthesis and Impact on Human Health"
9:40 am - 9:55 am
Discussion
9:55 am - 10:25 am
Coffee Break
10:25 am - 10:55 am
Valakunja Nagaraja (Indian Institute of Science, India)
"Targeting Topoisomerase I of M. tuberculosis with Small Molecule Inhibitors"
10:55 am - 11:10 am
Discussion
11:10 am - 11:40 am
Yuk-Ching Tse-Dinh (Florida International University, USA)
"Targeting Bacterial Topoisomerase I as Countermeasure for Antibiotic Resistance"
11:40 am - 11:55 am
Discussion
11:55 am - 12:05 pm
Maria Mills (National Heart, Lung, and Blood Institute, NIH, USA)
"Direct Observation of Type IA Topoisomerase Gate Opening"
12:05 pm - 12:10 pm
Discussion
12:10 pm - 12:20 pm
Matthew Hobson (Johns Hopkins School of Medicine, USA)
"A Mechanistic Framework for DNA Gyrase: DNA Wrapping Thermodynamics Governs Supercoiling Efficiency in E. coli Gyrase"
12:20 pm - 12:25 pm
Discussion
12:25 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
3:00 pm - 4:00 pm
Power Hour
The GRC Power Hour is an optional informal gathering open to all meeting participants. It is designed to help address the challenges women face in science and support the professional growth of women in our communities by providing an open forum for discussion and mentoring.
Organizer: Caroline Austin (Newcastle University, United Kingdom)
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Anticancer Drugs Targeting Topoisomerases: The Challenge to Make Them Better
Discussion Leader: Giovanni Capranico (University of Bologna, Italy)
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:10 pm
Scott Kaufmann (Mayo Clinic, USA)
"Topoisomerase I Poisons and DNA Repair Inhibitors: Improved Efficacy or Enhanced Toxicity?"
8:10 pm - 8:25 pm
Discussion
8:25 pm - 8:55 pm
Anish Thomas (Center for Cancer Research, National Cancer Institute, NIH, USA)
"Targeted Delivery of Topoisomerase Inhibitors: Are We There Yet?"
8:55 pm - 9:10 pm
Discussion
9:10 pm - 9:20 pm
Yasuki Kamai (Daiichi Sankyo Co., Ltd., Japan)
"Update on Preclinical and Clinical Development of Topoisomerase I Inhibitor Exatecan-Derivative Based Antibody-Drug Conjugate (DXd-ADC)"
9:20 pm - 9:25 pm
Discussion
9:25 pm - 9:30 pm
General Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Topoisomerases and Chromatin Structure
Discussion Leader: Andrzej Stasiak (University of Lausanne, Switzerland)
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:40 am
Andres Canela (National Cancer Institute, NIH, USA)
"Genome Organization Drives Chromosome Instability"
9:40 am - 9:55 am
Discussion
9:55 am - 10:25 am
Coffee Break
10:25 am - 10:55 am
Camilla Björkegren (Karolinska Institutet, Sweden)
"DNA Supercoiling and the Smc5/6 Complex"
10:55 am - 11:10 am
Discussion
11:10 am - 11:40 am
Caroline Austin (Newcastle University, United Kingdom)
"The Role of Topoisomerase II Beta (TOP2B) in Transcriptional Regulation"
11:40 am - 11:55 am
Discussion
11:55 am - 12:05 pm
Monica Guo (Massachusetts Institute of Technology, USA)
"A Bacterial Chromosome Structuring Protein Stimulates Type II Topoisomerases and Enables DNA Replication"
12:05 pm - 12:10 pm
Discussion
12:10 pm - 12:20 pm
Anu Hangas (University of Eastern Finland, Finland)
"Ciprofloxacin Impairs Mitochondrial DNA Replication Initiation Through Inhibition of Topoisomerase 2"
12:20 pm - 12:25 pm
Discussion
12:25 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Topoisomerases, Transcription and RNA Topoisomerases
Discussion Leader: James Berger (Johns Hopkins School of Medicine, USA)
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:10 pm
Weidong Wang (National Institute on Aging, NIH, USA)
"Evidence that Topoisomerase III Beta (TOP3B) Is a Dual Activity Topoisomerase that Can Act on both DNA and RNA"
8:10 pm - 8:25 pm
Discussion
8:25 pm - 8:55 pm
David Levens (National Cancer Institute, NIH, USA)
"The Interplay Between DNA Structure, Torsional Stress and Transcription in Mammalian Cells"
8:55 pm - 9:10 pm
Discussion
9:10 pm - 9:20 pm
Heeyoun Bunch (Kyungpook National University, South Korea)
"DNA Break and Damage Response Signaling Coupled with RNA Polymerase II Promoter-Proximal Pause Release and Processive Elongation"
9:20 pm - 9:25 pm
Discussion
9:25 pm - 9:30 pm
General Discussion
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Topoisomerase-Induced Damage
Discussion Leader: Brigitta Knudsen (Aarhus University, Denmark)
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:40 am
Sue Jinks-Robertson (Duke University Medical Center, USA)
"Topoisomerase I as a Driver of Genetic Change in Yeast"
9:40 am - 9:55 am
Discussion
9:55 am - 10:25 am
Coffee Break
10:25 am - 10:55 am
Shunichi Takeda (Kyoto University, Japan)
"DNA Repair Proteins Involved in the Repair of Pathological Topoisomerase II Cleavage Complexes"
10:55 am - 11:10 am
Discussion
11:10 am - 11:40 am
John Nitiss (University of Illinois at Chicago, USA)
"Regulation of the Repair of Topoisomerase II Covalent Complexes"
11:40 am - 11:55 am
Discussion
11:55 am - 12:05 pm
Sébastien Britton (Institute of Pharmacology and Structural Biology, CNRS, France)
"Mechanisms Antagonizing Ku at Single-Ended DNA Double-Strand Breaks Induced by Topoisomerase I Poisons"
12:05 pm - 12:10 pm
Discussion
12:10 pm - 12:20 pm
Hong Yan (Fox Chase Cancer Center, USA)
"Alternative End Joining Is a Major Pathway for the Repair of Top2-Mediated DSBs"
12:20 pm - 12:25 pm
Discussion
12:25 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:00 pm - 7:30 pm
Business Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
7:30 pm - 9:30 pm
Repair of Topoisomerase-Induced Damage
Discussion Leader: Felipe Cortés-Ledesma (CABIMER, CSIC, Spain)
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:10 pm
Scott Williams (National Institute of Environmental Health Sciences, NIH, USA)
"Mechanisms of TOP2 DNA-Protein Crosslink Repair"
8:10 pm - 8:25 pm
Discussion
8:25 pm - 8:55 pm
Shar-Yin Huang (Center for Cancer Research, National Cancer Institute, NIH, USA)
"Topoisomerase Damage and Repair in Mitochondria"
8:55 pm - 9:10 pm
Discussion
9:10 pm - 9:20 pm
Benu Das (Indian Association for the Cultivation of Science, India)
"PRMT5-Mediated Arginine Methylation of TDP1 for the Repair of Topoisomerase I Covalent Complexes"
9:20 pm - 9:25 pm
Discussion
9:25 pm - 9:30 pm
General Discussion
Thursday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Topoisomerases and Human Diseases (Neurodegenerative Diseases and Cancers)
Discussion Leader: Valerie Lamour (Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), France)
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:40 am
Peter McKinnon (St. Jude Children's Research Hospital, USA)
"Pathogenic Topoisomerase I Activity in Neurologic Disease"
9:40 am - 9:55 am
Discussion
9:55 am - 10:25 am
Coffee Break
10:25 am - 10:55 am
Sherif El-Khamisy (University of Sheffield, United Kingdom)
"Neurodegeneration at the Interface Between DNA Repair and Autophagy"
10:55 am - 11:10 am
Discussion
11:10 am - 11:40 am
Ram Madabhushi (University of Texas Southwestern Medical Center, USA)
"The Role of Activity-Induced DNA Breaks in Neuronal Physiology and Disease"
11:40 am - 11:55 am
Discussion
11:55 am - 12:05 pm
Thomas Nicholls (University of Gothenburg, Sweden)
"Topoisomerase 3a Is Required for Separation of Human Mitochondrial DNA"
12:05 pm - 12:10 pm
Discussion
12:10 pm - 12:20 pm
Laura Baranello (Karolinska Institutet, Sweden)
"Direct Regulation of Topoisomerase Activity as a Mean to Control Transcription"
12:20 pm - 12:25 pm
Discussion
12:25 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Tyrosyl DNA Phosphodiesterases, Recombinations, Mitochondrial and Viral Replication
Discussion Leader: Heidrun Interthal (Institute of Cell Biology, University of Edinburgh, United Kingdom)
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:10 pm
Keith Caldecott (University of Sussex, United Kingdom)
"Tyrosyl DNA Phosphodiesterase (TDP) Proteins in DNA Repair and Disease"
8:10 pm - 8:25 pm
Discussion
8:25 pm - 8:55 pm
Michael Weinfeld (University of Alberta, Canada)
"The Role of Polynucleotide Kinase/Phosphatase in DNA Repair and Its Potential as a Therapeutic Target"
8:55 pm - 9:10 pm
Discussion
9:10 pm - 9:20 pm
Dagmar Klostermeier (University of Muenster, Germany)
"Insights into the Mechanisms of DNA Supercoiling, Relaxation and Decatenation by Type II Topoisomerases"
9:20 pm - 9:25 pm
Discussion
9:25 pm - 9:30 pm
General Discussion
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure