| Session 1 |
Sunday p.m. |
| New 3D Structures of Membrane Proteins |
- Thue W. Schwartz: Introduction
- Doug Rees: X-ray structure of a pentameric mechanosensitive channel from M. tuberculosis
- Janos K. Lanyi: Molecular gating at 1.55 Å in bacteriorhodopsin
- Yoshinori Fujiyoshi: Aquaporin and other membrane proteins by helium temp EM
| Session 2 |
Monday a.m. |
| Multiple Molecular Mechanisms of Gating |
Discussion Leader: Wayne Hubbell
- Eduardo Perozo: Structural rearrangements in proton-dependent K+ channel gating.
- Pancho Bezanilla: Molecular mechanism of voltage gating in K+ channels.
- Horst Vogel: Probing single ligand-gated ion channel molecules by combined electrical and optical measurements.
- David Julius: Capsaisin receptor and heat activated channels.
Poster session A (Monday afternoon 4:00 - 6:00)
| Session 3 |
Monday p.m. |
| Translocation and Solubilization of Proteins - in and out of Membranes |
Discussion Leader: Stephen White
- Alan Finkelstein: Protein translocation associated with voltage gating of toxin channels.
- Harry Haigler: Soluble annexin forms transmembrane channel by reversible "inside-out" refolding.
- Jean-Luc Popôt: Amphipols: how to handle integral membrane proteins in detergent-free aqueous solutions.
| Session 4 |
Tuesday a.m. |
| Protein-Protein Interactions in the Membrane |
Discussion Leader: Don Engelman
- Don Engelman: Helix interactions in vivo - better physical chemistry through genetics.
- John Spudich: A 7-helix phototaxis receptor - conformational changes couple signaling to a co-receptor.
- Fiona Marshall: Surface expression of 7TM receptors through 1TM-7TM and 7TM-7TM heterodimerization.
- Mark Lemon: Ligand-induced homo- and hetero-dimerization of growth factor receptor tyrosine kinases.
- Four short presentations from posters ( Discussion Leader: Diane Papaizin)
- Paula Booth
- Thomas J. Baranski
- Anne Dam Jensen
- Isabelle Pellerin
Poster session B (Tuesday afternoon 4:00 - 6:00)
| Session 5 |
Tuesday p.m. |
| Forces and Principles in Ligand Recognition |
Discussion Leader: Barry Honig
- Barry Honig: Principles and forces of ligand recognition
- Jim A.Wells: Molecular recognition at protein interfaces
- Christian Elling: Metal-ion mediated ligand binding - 7TM receptor activation.
- Dennis A. Dougherty: Receptor side chains and backbone in ligand recognition and gating of the nicotinic receptor
| Session 6 |
Wednesday a.m. |
| ß-barrel Membrane Proteins - and Aquaporin |
Discussion Leader: Tilman Schirmer
- Tilman Schirmer: Selective versus non-selective porins
- Jeremy Lakey: Ligand induced gating of OmpF porin beta-barrel ion channels
- Susan Buchanan: The ferric enterobactin receptor - a ß-barrel active transporter
- Kaspar Locher: Structure of the hydroximate receptor FhuA in its apo and holo form
- Peter Agre: Molecular mechanism of gating in aquaporins
Tour to the Getty Museum optional (Wednesday afternoon)
| Session 7 |
Wednesday p.m. |
| Transporters - Recognition and Gating |
Discussion Leader: Ron Kaback
- Michael P. Kavanaugh: Glutamate transport - as viewed from both the ligand and the transporter
- Ron Kaback: What to do while waiting for your protein to crystalize !
- Shimon Schuldiner: Structure and function of EmrE, the smallest multidrug resistance transporter
| Session 8 |
Thursday a.m. |
| Conformational Changes & Ligand Binding in 7TM Receptor |
Discussion Leader: Gebhard Schertler
- Gebhard Schertler: Structure of bovine rhodopsin obtained by cryo-microscopy: a first view of an inverse agonist bound to a G-protein couple receptor
- Wayne Hubbell: Rhodopsin - conformational changes of activation probed by EPR
- Jean-Luc Galzi: Simultaneous time-resolved recording of signaling and ligand-binding by FRET in 7TM's
- Falk Fahrenholtz: Cholesterol / steroid dependency of certain 7TMs
- Bruce Conklin: Controlled in vivo ligand recognition of 7TM's in transgenic mice
| Session 9 |
Thursday p.m. |
| De novo Design and Recognition in Membrane Proteins |
Discussion Leader: Bill DeGrado
- Bill Degrado: De novo design of membrane proteins
- Hagan Bayley: Redesign of alpha-hemolysin - ligand recognition / bioprobe
- Gerd Koechendorfer: Chemical synthetic approach to membrane proteins
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