Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
7:40 pm - 9:30 pm
Keynote Session: The AI-Driven Revolution in Structural Biology
Discussion Leader: Wah Chiu (Stanford University, United States)
7:40 pm - 7:55 pm
Opening Remarks
7:55 pm - 8:15 pm
Introduction by Discussion Leader
8:15 pm - 8:35 pm
Randy Read (University of Cambridge, United Kingdom)
"Experimental Structural Biology Post-AlphaFold: Exploiting its Strengths and Compensating for its Weaknesses"
8:35 pm - 8:45 pm
Discussion
8:45 pm - 9:05 pm
Mohammed AlQuraishi (Columbia University, United States)
"The State of Protein Structure Prediction and Friends"
9:05 pm - 9:15 pm
Discussion
9:15 pm - 9:30 pm
General Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Specimen Preparation Challenges and Solutions
Discussion Leader: Giovanna Scapin (Nanoimaging Services, United States)
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
Ashwin Chari (Max Planck Insitute for Multidisciplinary Sciences, Germany)
"Biochemical Challenges in Achieving High-Resolution in EM"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
David Veesler (University of Washington, United States)
"Structural Studies of Small Protein Complexes of Therapeutic Interest"
10:10 am - 10:25 am
Discussion
10:25 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
Andreas Engel (Case Western Reserve University, Switzerland)
"Preparing Vitrified Grids From Nano Litre Sample Volumes"
11:10 am - 11:25 am
Discussion
11:25 am - 11:45 am
Amelie Leforestier (Laboratoire de Physique des Solides, CNRS, University Paris-Saclay, France)
"Optimizing Thin Film and Cryo-Section Cryo-EM Sample Preparation"
11:45 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Radoslav Enchev (The Francis Crick Institute, United Kingdom)
"Development and Applications of Time-Resolved Cryo-EM Sample Preparation"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hour™
The GRC Power Hour™ is designed to address diversity and inclusion in the scientific workplace by providing a safe environment for informal and meaningful conversations amongst colleagues of all career stages. The program supports the professional growth of all members of our communities, including ethnicity, race and/or gender identity by providing an open forum for discussion and mentoring.
Organizer: Susan Hafenstein (University of Minnesota, Hormel Institute, United States)
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Cryo-Electron Tomography
Discussion Leader: Ariane Briegel (Leiden University, The Netherlands)
6:00 pm - 6:15 pm
Introduction by Discussion Leader
6:15 pm - 6:35 pm
Gaia Pigino (Fondazione Human Technopole, Italy)
"Molecular Transformers: Cryo-ET Reveals The Mechanism of Conversion From Anterograde to Retrograde Intraflagellar Trasport Trains"
6:35 pm - 6:50 pm
Discussion
6:50 pm - 7:10 pm
Elizabeth Villa (University of California, San Diego and Howard Hughes Medical Institute, United States)
"A Generalized Nanogold Tagging System For The Identification of Macromolecules Inside Cells"
7:10 pm - 7:25 pm
Discussion
7:25 pm - 7:45 pm
Christopher Ackerson (Colorado State University, United States)
"Cloneable Cellular EM Contrast Agents"
7:45 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Tuesday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
Image Processing and Computation
Discussion Leader: Steven Ludtke (Baylor College of Medicine, United States)
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
Carlos Oscar Sorzano (Spanish National Research Council (CSIC), Spain)
"Image Alignment Algorithms for Single Particle Analysis"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
Ali Punjani (Structura Biotechnology Inc. / University of Toronto, Canada)
"New Developments in CryoSPARC for Single Particle Data Processing"
10:10 am - 10:25 am
Discussion
10:25 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
Daisuke Kihara (Purdue University, United States)
"Building and Validating Structure Models from Medium to Low Resolution Cryo-EM Maps"
11:10 am - 11:25 am
Discussion
11:25 am - 11:45 am
Dari Kimanius (Chan Zuckerberg Imaging Institute, United States)
"Cryo-EM Reconstruction and Interpretation through Data-Driven, Differentiable Approaches"
11:45 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Beata Turonova (Max Planck Institute of Biophysics, Germany)
"Contextual Analysis For In Situ Cryo Electron Tomography"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Selected Poster Presentations
Discussion Leader: Abraham Koster (Leiden University Medical Center, The Netherlands)
6:00 pm - 6:10 pm
Mart Last (Leiden University Medical Centre, The Netherlands)
"Single-Molecule Fluorescence Localization Guided Cryo-Electron Tomography"
6:10 pm - 6:15 pm
Discussion
6:15 pm - 6:25 pm
Ernesto Arias Palomo (CIB Margarita Salas (CSIC), Spain)
"Molecular Basis for Transposase Activation by a Dedicated AAA+ ATPase"
6:25 pm - 6:30 pm
Discussion
6:30 pm - 6:40 pm
Melody Campbell (Fred Hutchinson Cancer Center, United States)
"De Novo Design of Highly Selective Miniprotein Inhibitors of Integrins avß6 and avß8"
6:40 pm - 6:45 pm
Discussion
6:45 pm - 6:55 pm
Yao Cong (Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, CAS, China)
"Development of Immobilized Antibody-Based Affinity Grid Strategy and Application in On-Grid Purification of Target Proteins for High-Resolution Cryo-EM"
6:55 pm - 7:00 pm
Discussion
7:00 pm - 7:10 pm
Marc Aurèle Gilles (Princeton University, United States)
"A Bayesian Framework for Cryo-EM Heterogeneity Analysis using Regularized Covariance Estimation"
7:10 pm - 7:15 pm
Discussion
7:15 pm - 7:25 pm
Gaia Perone (Human Technopole, Italy)
"SOLIST - A New Cryo-Lift Out Approach for a Biopsy At the Nanoscale"
7:25 pm - 7:30 pm
Discussion
7:30 pm - 7:40 pm
Mingyu Jung (Seoul National University, South Korea)
"Co-VET: Correlative Voltage Imaging and Cryo-Electron Tomography"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
Rasmus Kjeldsen Jensen (EMBL, Heidelberg, Germany)
"Novel Complex Discovery with In-Cell Cryo-ET & AlphaFold Exemplified by Identification of a Non-Canonical Bacterial Translocation Complex"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Novel Biological Advances
Discussion Leader: Lori Passmore (MRC Laboratory of Molecular Biology, United Kingdom)
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
Aashish Manglik (University of California, San Francisco, United States)
"Seeing Scents to Understand our Sense of Smell"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
Hao Wu (Harvard Medical School / Boston Children's Hospital, United States)
"Cryo-EM in Elucidating Supramolecular Inflammasome Complexes"
10:10 am - 10:25 am
Discussion
10:25 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
Wooyoung Choi (University of California, San Francisco, United States)
"Characterizing Large Domain Motions in Single Particle Cryo-EM Structures"
11:10 am - 11:25 am
Discussion
11:25 am - 11:45 am
Damian Ekiert (Johns Hopkins University, United States)
"Structural Cell Biology of an Emerging Human Pathogen"
11:45 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Roger Craig (UMass Chan Medical School, United States)
"Cryo-EM Structure of the Human Cardiac Myosin Filament"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Hardware and Workflow Advances
Discussion Leader: David A. Muller (Cornell University, United States)
6:00 pm - 6:15 pm
Introduction by Discussion Leader
6:15 pm - 6:35 pm
Ulrich Lorenz (Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland)
"Advances in Microsecond Time-Resolved Cryo-EM"
6:35 pm - 6:50 pm
Discussion
6:50 pm - 7:10 pm
Abhay Kotecha (Thermo Fisher Scientific, The Netherlands)
"In Situ Visual Proteomics and Molecular Tissue Imaging with PlasmaFIB and Electron Cryo-Tomography"
7:10 pm - 7:25 pm
Discussion
7:25 pm - 7:45 pm
Angus Kirkland (The Rosalind Franklin Institute, United Kingdom)
"Electron Ptychographic Methods at Low Dose: Making Every Electron Count"
7:45 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair(s); Complete the GRC Evaluation Forms; Election of the Next Vice Chair(s)
9:00 am - 12:30 pm
Integrative Methods
Discussion Leader: Grant Jensen (Brigham Young University, United States)
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
Bruno Klaholz (CBI/IGBMC, France)
"Human Ribosome Structures at Resolutions Better Than 2 Å"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
Pilar Cossio (Flatiron Institute, United States)
"Conformational Heterogeneity & Validation Using MD Simulations and Cryo-EM"
10:10 am - 10:20 am
Discussion
10:20 am - 10:45 am
Coffee Break
10:45 am - 11:05 am
Juri Rappsilber (Technische Universität Berlin, Germany)
"High-Contrast Crosslink Mass Spectrometry as Input for AlphaLink for In-Cell Structures"
11:05 am - 11:20 am
Discussion
11:20 am - 11:40 am
Lucy Collinson (Francis Crick Institute, United Kingdom)
"Connecting Volume EM and In Situ Structural Biology to Imaging Across Scales"
11:40 am - 11:55 am
Discussion
11:55 am - 12:15 pm
Wesley Legant (University of North Carolina at Chapel Hill, United States)
"Lattice Light Sheet Microscopy - Innovations, Applications, and Future Directions"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Selected Poster Presentations
Discussion Leader: Jenny Hinshaw (NIH, United States)
6:00 pm - 6:10 pm
Joshua Dickerson (MRC Laboratory of Molecular Biology, United Kingdom)
"Liquid Helium Specimen Temperatures for CryoEM Part 2: Understanding and Eliminating Beam-Induced Motion"
6:10 pm - 6:15 pm
Discussion
6:15 pm - 6:25 pm
Vasilii Mikirtumov (MDC Berlin, Germany)
"Structure of RyR1 in Native Membrane by Cryo-Electron Tomography"
6:25 pm - 6:30 pm
Discussion
6:30 pm - 6:40 pm
Xinzheng Zhang (Institute of Biophysics,Chinese Academy of Sciences, China)
"Determining Protein Structures and Dynamic Process in Cellular Lamellae at Pseudo-Atomic Resolution by GisSPA"
6:40 pm - 6:45 pm
Discussion
6:45 pm - 6:55 pm
Hamish Brown (The University of Melbourne, Australia)
"In Cryo-EM it's Hip to Be Square: Development of Novel Condenser Apertures for Square and Rectangular Illumination for Single Particle Cryo-EM"
6:55 pm - 7:00 pm
Discussion
7:00 pm - 7:10 pm
Anna Sundborger-Lunna (Uppsala University, Sweden)
"Mechanisms of BAR-Mediated Membrane Remodelling"
7:10 pm - 7:15 pm
Discussion
7:15 pm - 7:25 pm
Mario Borgnia (National Institutes of Health, United States)
"Expanding the Reach of Cryo-EM Through Open Design Robotics for Specimen Preparation and Secure Multi Platform Remote Microscope Operation"
7:25 pm - 7:30 pm
Discussion
7:30 pm - 7:40 pm
Rahel Woldeyes (Stanford University, United States)
"Cryo-Electron Tomography Reveals the Structural Diversity of Cardiac Proteins in their Cellular Context"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
Ellen Zhong (Princeton University, United States)
"CryoBench: Datasets and Benchmarks for Heterogeneous Cryo-EM Reconstruction"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure